AMBICIN® L 溶葡球菌酶

AMBICIN L Lysostaphin （订货以CAS编码或英文名称为准）

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编号：AMBI-LSPN

品牌：AMBI

分子式：C16H14N2O3

CAS号：9011-93-2

分子量：282.29

申请试用

中文名称：

溶葡球菌酶

英文名称：

AMBICIN L Lysostaphin

CAS号：

9011-93-2

分子式：

C16H14N2O3

分子量：

282.29

详细描述

品牌介绍：

AMBI公司自1991年以来一直以AMBICIN®L的商品名生产和销售的重组溶葡球菌酶，纯度超过90%。AMBICIN® L 溶葡球菌酶是一种高度纯化的重组溶葡萄球菌酶制剂，是研究葡萄球菌流行病学和分子遗传学的重要工具。AMBICIN L是最具成本效益的溶葡萄球菌酶，是具有较高成本优势的溶葡萄球菌素。

AMBICIN® L 溶葡球菌酶是葡萄球菌细胞壁肽聚糖的特异性内肽酶，是一种非常有效的抗葡萄球菌药物。克隆的溶葡萄球菌酶基因是AMBI专利主体。

AMBICIN® L 溶葡球菌酶被用作研究和诊断工具。由于它能有效地裂解葡萄球菌，因此在制备用于遗传和生化研究的葡萄球菌DNA或其他细胞成分以及制备用于转化的原生质体时被广泛使用。在细菌DNA的制备和分析中，已成为临床和其他微生物学家追踪感染或细菌污染源的流行病学研究中使用的有力工具。

产品详细介绍：

中文名称：AMBICIN® L 溶葡球菌酶
CAS号：9011-93-2
中文别名：AMBI溶葡球菌酶
英文名称：Lysostaphin
分子式：C16H14N2O3
分子量：282.294

Specific Activity: > 3,990 units/mg

Peptide Purity: > 93%

Trade name: AMBICIN L
Chemical Family: Enzyme (Protein antibacterial)

PHSYICAL DATA
	Appearance:	Amorphous off-white powder
	Odor:	None
	Solubility:	> 15 mg/ml in 20 mM Na acetate pH 4.5
	Isoelectric Point	10.4 - 11.4

Gene:

Lysostaphin gene from Staphylococcus simulans, Genbank accession numbers M15686 and I00376

Expression organism:

E. coli

Activity assay:

Determined by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus 8325-4 at pH 8.0, 30° C (AMBI Protocol TP-031). Specific activity is measured relative to an AMBI lysostaphin standard with a defined activity of 4,000 U/mg

Storage conditions:

Long-term:	Store in sealed container with desiccant at -20° C.
Solution:	Stable for at least 6 months when stored at 4°C as 10 mg/ml solution in 20 mM sodium acetate, pH 4.5, in a polypropylene container.

Method of analysis:

Reverse phase HPLC monitored at 210 nm (AMBI Protocol TP-112.M)

Peptide purity:

>90% (reverse phase HPLC)

mg protein/mg solid:

>0.9 (Absorbance at 280 nm; extinction coefficient = 2.02 AU/mg/ml)

Additives:

None

Specific activity:

>3,000 units/mg (Turbidometric assay AMBI Protocol TP-031)

Physical form:

Lyophilized powder

Shelf life:

<1% loss of activity per year, when stored dry at -80° C

Batch Information

Lot Number:

GDV 1

Specific activity:

3552 units/mg

Peptide purity:

93.4% (HPLC, % of peak area)

References

Recsei, P.A. (June 5, 1990) U.S. Pat. # 4931390.

Recsei, P.A., Gruss, A.D., and Novick, R.P. (1987) Cloning, sequence, and expression of the lysostaphin gene from Staphylococcus simulans. Proc. Nat. Acad. Sci., U.S.A. 84: 1127-1131.

Kline, S.A., J. de la Harpe, and P. Blackburn. (1994). A colorimetric microtitre plate assay for lysostaphin using a hexaglycine substrate. Anal. Bioch. 217, 329-331.

Schindler, C.A. and V.T. Schuhardt. (1964) Lysostaphin: a new bacteriolytic agent for the stapylococci.Proc. Nat. Acad. Sci. U.S.A. 51: 414-421.

Novick, R. P. (1991). Methods in Enzymology 204:587-636

Goering , R.V and M. A. Winters. (1992). Rapid method for epidemiological evaluation of Gram-positive cocci by field inversion gel electrophoresis. J. Clin. Microbiol. 30:577-580

Bannerman, T. L., G. A. Hancock, F. C. Tenover and J. M. Miller. (1995). Pulsed-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus. J. Clinical Microbiol. 33:551-555

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