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创赛优选商城提供的[Sigma-Aldrich]4-Nitrophenyl N-Acetyl-β-D-Glucosaminide为创赛科技出品,产品稳定,定制速度快,极大地方便了广大科研客户的使用需求。产品包装为500MG,安全运输。
属性:
质量水平:300
测定:≥99% (TLC)
形式:powder
杂质:≤0.05% Free p-nitrophenol
溶解性:water: 5 mg/mL, clear, colorless to very faintly yellow
储存温度:−20°C
SMILES string:CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc(cc2)[N+]([O-])=O
InChI:1S/C14H18N2O8/c1-7(18)15-11-13(20)12(19)10(6-17)24-14(11)23-9-4-2-8(3-5-9)16(21)22/h2-5,10-14,17,19-20H,6H2,1H3,(H,15,18)/t10-,11-,12-,13-,14-/m1/s1
InChI key:OMRLTNCLYHKQCK-DHGKCCLASA-N
应用:
N-acetyl-β-D-glucosaminidase (NAG) has been used:
as a substrate to assay N-acetyl-β-D-glucosaminidase (NAG)
as a substrate to study chitinase ChiB1 activity
to assess degranulation using rat basophilic leukemia (RBL)-2H3 cells
包装:
100, 250, 500 mg in poly bottle
1 g in poly bottle
生化/生理作用:
4-Nitrophenyl N-acetyl-β-D-glucosaminide (NP-GlcNAc) is a widely used hexosaminidase substrate. Enzymatic action cleaves the glycosidic bond and forms 4-nitrophenolate (pNP) which is measured at 405 nm. NP-GlcNAc can also be used in combination with diethylaminoethyl-a-cyclodextrin (DEn-CD) for a rapid and accurate rate assay method for b-N-acetylglucosaminidase. The cyclodextrin derivative is used as an additive to ionize 4-nitrophenol to yellow-colored 4-nitrophenoxide at a pH near 5, the pH optimum for the enzyme.
底物:
N-乙酰-氨基葡糖苷酶的发色底物。
查询关键词:“SIGMA-N9376,3459-18-5,4-硝基苯基-N-乙酰-β-D-氨基葡糖苷,4-Nitrophenyl N-Acetyl-β-D-Glucosaminide,Sigma-Aldrich,上海现货”。
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